ABSTRACT
OBJECTIVE: The aim of this study was to evaluate the effect of oral tamoxifen treatment on the number of myofibroblasts present during the healing process after experimental bile duct injury. METHODS: The sample consisted of 16 pigs that were divided into two groups (the control and study groups). Incisions and suturing of the bile ducts were performed in the two groups. Tamoxifen (20 mg/day) was administered only to the study group. The animals were sacrificed after 30 days. Quantification of myofibroblasts in the biliary ducts was made through immunohistochemistry analysis using anti-alpha smooth muscle actin of the smooth muscle antibody. Immunohistochemical quantification was performed using a digital image system. RESULTS: In the animals treated with tamoxifen (20 mg/day), there was a significant reduction in immunostaining for alpha smooth muscle actin compared with the control group (0.1155 vs. 0.2021, p = 0.046). CONCLUSION: Tamoxifen reduced the expression of alpha smooth muscle actin in the healing tissue after bile duct injury, suggesting a decrease in myofibroblasts in the scarred area of the pig biliary tract. These data suggest that tamoxifen could be used in the prevention of biliary tract stenosis after bile duct surgeries.
Subject(s)
Animals , Female , Bile Ducts/injuries , Estrogen Antagonists/therapeutic use , Myofibroblasts/drug effects , Tamoxifen/therapeutic use , Wound Healing/drug effects , Actins/analysis , Actins/drug effects , Bile Ducts/drug effects , Cell Count , Immunohistochemistry , Muscle, Smooth/chemistry , Muscle, Smooth/drug effects , Reproducibility of Results , Swine , Treatment OutcomeABSTRACT
O presente estudo teve como objetivo avaliar alteraçöes na pressäo arterial média (PAM) com uso de bupivacaína hiperbárica 0,5 por cento (0,3 mg.kg(-1)) no espaço subaracnóide de cäes. Foram utilizados seis animais, fêmeas, sem raça definida, na faixa etária entre um a cinco anos, hígidas e com peso variando entre sete e 15 kg. Após a contençäo mecânica dos animais foi realizada a punçäo do ramo cranial da artéria safena com o intuito da aferiçäo basal da pressäo arterial média (PAM). Os animais foram pré-oxigenados com O2 a 100 por cento (3 L/min) durante 5 minutos, induzidos com propofol (6 mg/kg) e, após a perda dos reflexos protetores, foi realizada a segunda aferiçäo da PAM. A terceira mensuraçäo da PAM procedeu-se após a intubaçäo orotraqueal. Os animais foram monitorados e mantidos através de infusäo contínua de propofol (0,5mg.kg(-1).min) e a PAM mensurada durante 15 minutos em intervalos de 5 minutos. Posteriormente, foram realizadas a anti-sepsia e assepsia da regiäo lombossacra e o espaço subaracnóide puncionado (L6-L7), para administraçäo de bupivacaína (0.3 mg.kg(-1)). Uma vez constatado o bloqueio, a PAM foi mensurada durante um período de uma hora, com intervalos de 5 minutos. Como conclusäo deste estudo näo observamos nenhum valor pressórico compatível com um quadro hipotensivo.